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The investigators surgically detached the Achilles tendon from its calcaneal insertion in rats an injury that does not heal spontaneously and assessed recovery with and without BPC-157 administration

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The % of cytotoxicity was measured using the following formula: Click-iT plus TUNEL assay To perform TUNEL Alexa Fluor Staining, the commercial Click-iT TUNEL Alexa Fluor Imaging Assay (C10247, Invitrogen , Warsaw, Poland) was used in accordance with the instructions provided by the manufacturer